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DGENE, USGENE, and PCTGEN: new percent identity feature for sorting BLAST answer sets

30. November 2009

A new sequence sorting feature for BLAST answer sets from DGENE, USGENE and PCTGEN is available since November 30, 2009. This new sorting option is a great support for reviewing large sequence answer sets and makes the identification of relevant sequences much more efficient and reliable. The enhanced sorting functionality allows you to sort a BLAST answer set according to local percent identity (IDENT). Additional sorting with this BLAST parameter is especially useful to identify short, highly similar sequences with a low overall homology score.

The new percent identity field IDENT is available for sorting and display purposes. Ideally it can be used in combination with the conventional homology sorting parameter SCORE. Using both parameters simultaneously for sorting generates answer sets which are more easy to review. For this kind of dual sorting either of the two BLAST parameters can be specified to be the premier sort parameter. On top of this, customers have the choice to group sequence records into invention families using the FSORT command. When FSORT is applied to an answer set previously sorted by IDENT and SCORE, the sort order is retained for each family. Thus the new sorting option adds additional flexibility to the display of BLAST answer sets.

The new sorting feature is available for all online and batch BLAST search results, and also for BLAST alert results in all three databases.

Read the full article with search and display examples at:
www.stn-international.de/percent_identity_sorting.html

The revised summary sheets are available at:
http://www.stn-international.de/sum_sheets.html